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Jupyter notebook 22_Prelab_Pharmacology/Prelab_pharmacology.ipynb

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Kernel: Python 2 (SageMath)

Pre-lab: Bioinformatics in Pharmacology

I. Recorded Lecture

Head to Canvas and review the slides and watch the recorded lecture "Bioinformatics in Pharmacology" (recorded by John Hogenesch). Then, answer the following questions.

Q1. What gene has the most publications associated with it (as of 2007)? Do most genes have a lot of publications assoicated with them? Why do you think that is?

P53. No, there are only few publications associated with each genes. Its because of funding is more likely to be available for known genes

Q2. Describe two genetics-based strategies that could be used to identify new, potential therapeutic drug targets for disease.

Forward genetics GWAS

Q3. What is the Z' factor? In what context is it utilized, and how?

measure of assay performance. It is used measure actual signal vs noise and variation. It is calculated by multiplying the the sum of standard deviationof positive and negative controls x 3 divided by difference in mean of positive and negative control. Should be greater than 1 to say a good assay.

II. Cell-based screening

For our activity for this unit, we have decided to focus on the analysis of data from high-throughput cell screening data. In industry, this is routinely done, for drug discovery, target identification, lead compound optimization, assay development, and more. On Canvas, we have provided you two papers (as some brief overview) to describe the process, features, misconceptions, and examples. See Szymański et al (2012) and Macarron et al (2011).

However, the analysis of these data requires data management and analytical processing at large input: hundreds, perhaps thousands of plates to analyze, and something that you can not readily do in excel, even though many of the calculations are relatively straight forward.