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Week 2: Pipet Challenge and Jupyter Notebook Workflow

Group ID: MFZ

Group Members: Maggie Morse, Fernanda Ribas, Zunaira Arshad

I. Lab Objective

The objective for this lab is to explore our pipetting skills while also discovering how to use Jupyter.

II. Methods

Part 1

  1. We gathered the materials:
    Yellow colored water
    Blue colored water
    1.5 mL eppendorf tube
    P1000, P200, P20 and corresponding tips
    Plastic beaker for used tips
    Tube rack

  2. We put the eppendorf tube in the tube rack and opened it

  3. We used the P1000 pipette and corresponding tip to transfer 1000μL of yellow colored water into the test tube
    We used the P1000 pipette and corresponding tip to transfer 400μL of blue colored water into the test tube
    We used the P200 pipette and corresponding tip to transfer 150μL of yellow colored water into the test tube
    We used the P200 pipette and corresponding tip to transfer 50.5μL of blue colored water into the test tube
    We used the P1000 pipette and corresponding tip to transfer 378μL of yellow colored water into the test tube
    We used the P20 pipette and corresponding tip to transfer 21.5μL of blue colored water into the test tube

  4. We recorded the settings as they appeared in the windows othe pipettes

  5. We recorded the number of microlitres in the tube

  6. We recorded the number of millilitres in the tube

  7. We compared our test tube to our classmates' test tubes

Part 2

  1. We weighed four eppendorf tubes and recorded their masses

  2. We pipetted 1000μL of blue liquid into each of the 4 tubes

  3. We weighed the four eppendorf tubes filled with liquid

  4. We calculated the difference in weight to test the accuracy of our pipetting

III. Data Collection Narrative and Data Collected

Our data was collected on the worksheets for the two Micropipette Challenges. Data for Challenge Part I and II were entered into a comma-delimited .csv file, named "XXX"

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%matplotlib inline
#%load_ext rmagic
import matplotlib.pyplot as plt
import numpy as np
import pandas as pd
import pylab
%matplotlib inline
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pipette2 = pd.read_csv("Week2_Challenge2_GroupTA.csv")
#show data
pipette2

IV. Data Analysis Narrative

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V. Conclusions

VI. Pitfalls and Future Directions

One pitfall of our experiment was the size of the eppendorf tube used. We pipetted 2mL of liquid into a 1.5mL tube, making the process difficult and hard to keep from spilling.

For a future experiment, each individual should pipette liquid into separate eppendorf tubes in Part II to test each group members' pipetting accuracy.

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